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  • Plasmid Maxi Prep Column
  • Plasmid Maxi Prep Column
  • Plasmid Maxi Prep Column
  • Plasmid Maxi Prep Column

Plasmid Maxi Prep Column

biocomma® Plasmid Midi-Prep Column consists of a spin column and a collection tube. Utilizing the alkaline lysis method and the principle of silica membrane-specific binding to plasmid DNA, it enables rapid preparation of up to 500 μg of plasmid DNA.Purified plasmids are suitable for digestion, transformation, PCR, sequencing, and library construction.

Details
Features
● Plasmid DNA yield up to 500 μg
● Simple operation, high extraction efficiency
● High nucleic acid purity, suitable for various downstream applications

Experimental results:
Table 1:NP500 Plasmid yield
Name A260/280 Concentration(ng/μL) Yield (μg)
NP500 1.95 300 300
NP500 1.91 337 320
Collect 120 mL of overnight culture (DH5α host, pUC19 plasmid), extract the plasmid using NP500, with an elution volume of 1.0 .mL.


Ordering Information
Cat.# Description Volume Yield Qty
NP500 biocomma® Plasmid Maxiprep Columns 50 mL,22 mL ~500 μg 20pcs/pk
Note: Recommend 100-200 mLbacterial liquid and 1.0-2.0 mL elution solution for this column.

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