Plasmid Maxi Prep Column
biocomma® Plasmid Midi-Prep Column consists of a spin column and a
collection tube. Utilizing the alkaline lysis method and the principle of silica
membrane-specific binding to plasmid DNA, it enables rapid preparation of
up to 500 μg of plasmid DNA.Purified plasmids are suitable for digestion,
transformation, PCR, sequencing, and library construction.
Details
Features● Plasmid DNA yield up to 500 μg
● Simple operation, high extraction efficiency
● High nucleic acid purity, suitable for various downstream applications
Experimental results:Table 1:NP500 Plasmid yield
| Name |
A260/280 |
Concentration(ng/μL) |
Yield (μg) |
| NP500 |
1.95 |
300 |
300 |
| NP500 |
1.91 |
337 |
320 |
| Collect 120 mL of overnight culture (DH5α host, pUC19 plasmid), extract the plasmid using NP500, with an elution volume of 1.0 .mL. |
Ordering Information
| Cat.# |
Description |
Volume |
Yield |
Qty |
| NP500 |
biocomma® Plasmid Maxiprep Columns |
50 mL,22 mL |
~500 μg |
20pcs/pk |
| Note: Recommend 100-200 mLbacterial liquid and 1.0-2.0 mL elution solution for this column. |
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